Genetic Diversity of Agronomic Traits in Rice (Oryza sativa L.) Using Several Colchicine Concentrations
Keywords:
Generative phase, vegetative phase, selection character, mutation, effective treatment
Abstract
This study aimed to evaluate the effects of various colchicine concentrations on the genetic diversity of agronomic traits in rice (Oryza sativa L.). The experiment was conducted at the Agricultural Modernization Application Center (BRMP) Gorontalo from January to April 2025, employing a two-factor factorial completely randomized design (CRD). The first factor was rice variety, comprising two levels: V1 (Inpago 13 Fortis) and V2 (Inpari Nutri Zinc), while the second factor was colchicine concentration with four levels: P0(control/0 ppm), P1 (750 ppm), P2(1000 ppm), and P3 (1250 ppm). Each treatment combination was replicated three times, resulting in 24 experimental units. Observed parameters included both vegetative and generative phases. Results indicated that colchicine did not exert a significant overall effect on either vegetative or generative traits; however, certain concentrations induced an increase in the number of spikelets in the Inpago 13 Fortis variety. In contrast, Inpari Nutri Zinc exhibited higher tolerance to colchicine, maintaining stable growth and morphology across all concentrations. These findings highlight that colchicine effectiveness is highly dependent on dosage and genotype response, emphasizing the need for optimal concentrations to induce mutations without causing toxic effects, thereby providing a foundation for rice mutation breeding.
References
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Manzoor, A., Ahmad, T., Bashir, M. A., Hafiz, I. A., & Silvestri, C. (2019). Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants, 8(7), 194.
Priyadi I. Get to know Rice Inpari Nutri Zinc (2021) http://cybex.pertanian.go.id/artikel/97852/
Sabana, A., Ernawiati, E., Priyambodo, P., & Agustrina, R. (2022). Induksi poliploid planlet pisang kepok batu dengan kolkisin pada media kultur jaringan. Organisms: Journal of Biosciences,2(1), 1-8.
Sahoo, S. A. (2018). Studies on Radiation Mutagenesis in Anther Cultures and Characterization of Induced Genetic Variability in Rice (Oryza sativa L.). Thesis, Odisha University of Agriculture and Technology.
Septiningsih, E. M., Pamplona, A. M., Sanchez, D. L., Neeraja, C. N., Vergara, G. V., Heuer, S., ... & Mackill, D. J. (2009). Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond. Annals of Botany, 103(2), 151-160.
Shu, G. Y. (2009). Induced plant mutations in the genomics era.
Singh, H. (2022). Morpho-physiological and molecular profiling of rice (Oryza sativa L.) genotypes for yield related traits under direct seeded conditions.KrishiKosh Repository, ICAR India.
Subekti, A., & Umar, A. (2023). Keragaman Enam Varietas Unggul Baru Padi Khusus Pada Lahan Sub Obtimal di Kalimatan Barat. Jurnal Pertanian Agros, 25(1), 514-520.
Suhartini, T., & Sutoro, S. (2007). Pengelompokan Plasmanutfah Spesies Padi Liar (Oryza spp.) Berdasarkan Peubah Kuantitatif Tanaman. Berita Biologi, 8(6), 445-453.
Sujipuli, K., Inthima, P., Yimtragool, N., Warnnissorn, N., Warnnissorn, P., & Prasarnpun, S. (2021). In vitro colchicine-induced polyploids from different explant segments of Bacopa monnieri. ScienceAsia, 47(1).
Sumiasih, I. H. (2014). Pengaruh Lama Perendaman Beberapa Varietas Padi dengan Colchicine terhadap Komponen Agronomi dan Produksi. Skripsi. Universitas Jember.
Surson, S., Sitthaphanit, S., Prachachit, J., Jitjak, T., & Wongkerson, K. (2024). Effects of colchicine on polyploid induction, morphology, and yield components of several Thai rice varieties. Biodiversitas Journal of Biological Diversity, 25(12).
Surson, S., Sitthaphanit, S., Prachachit, J., Jitjak, T., & Wongkerson, K. (2025). Effects of Colchicine on the Morphology and Agricultural Characteristics of RD. 43 Thai Rice Variety. Indian Journal of Agricultural Research, 59(1).
Wang, Y., Zhang, H., Gigant, B., Yu, Y., Wu, Y., Chen, X., ... & Knossow, M. (2016). Structures of a diverse set of colchicine binding site inhibitors in complex with tubulin provide a rationale for drug discovery. FEBS Journal, 283(1), 102-111.
Wu, L., Zhang, W., Ding, Y., Zhang, J., Cambula, E. D., Weng, F., ... & Li, G. (2017). Shading contributes to the reduction of stem mechanical strength by decreasing cell wall synthesis in japonica rice (Oryza sativa L.). Frontiers in plant science, 8, 881.
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Zhao, S., Jang, S., Lee, Y. K., Kim, D. G., Jin, Z., & Koh, H. J. (2020). Genetic basis of tiller dynamics of rice reveale
Adil, M., Tahir, M., Sadiq, I., & Ahmad, I. (2019). Effect of colchicine on morphological traits of different plant species: A review. Pure and Applied Biology, 8(1), 343–351. https://doi.org/10.19045/bspab.2019.80026
Agrawal, P. K., Gosal, S. S., & Sidhu, G. S. (2001). Induction of bacterial blight resistance in elite Indian rice cultivars using gamma-rays and ethyl methanesulfonate.
Aliyu, R., Bayero, U., Adebola, M., Aminu, A., & Adamu, A. (2016). Mutagenic Effects Of Urea And Colchicine On Two Rice Landraces (Farrar Jollof And Yar Zamfara) At M1 Generation.
Ara, I. (2019). Enhancement of Abiotic Stress Tolerance in Rice (Oryza sativa L.) through Androgenesis and Transgenic Approaches. Institute of Biotechnology, Bangladesh.
Badan Pusat Statistik (BPS). (2024). Produksi Tanaman Padi Provinsi Gorontalo Tahun 2024. Gorontalo: BPS Provinsi Gorontalo.
Barus, J., Mustikawati, D. R., Arief, R. W., & Endriani. (2024, February). Effectiveness of bio-silica and bio-fertilizer on nutrient uptake in leaves and yield of rice inpari IR nutri-zink variety. In AIP Conference Proceedings (Vol. 2957, No. 1, p. 100001). AIP Publishing LLC.
Bhattacharyya, B., Panda, D., Gupta, S., & Banerjee, M. (2008). Anti-mitotic activity of colchicine and the structural basis for its interaction with tubulin. Medicinal Research Reviews, 28(1), 155-183.
Boy, R., & Soeharsono. (2013). Inventarisasi dan Identifikasi Sumberdaya Genetik Tanaman Padi di Kabupaten Banggai. Prosiding Seminar Nasional Sumber Daya Genetik Pertanian (pp. 126-133).
Brown, K. H., Peerson, J. M., Rivera, J., & Allen, L. H. (2002). Effect of supplemental zinc on the growth and serum zinc concentrations of prepubertal children: a meta-analysis of randomized controlled trials. The American journal of clinical nutrition, 75(6), 1062-1071.
Dwimahyani, I., & Dewi, W. S. (2010). Induksi mutasi tanaman padi (Oryza sativa L.) dengan iradiasi sinar gamma dan perlakuan kolkisin. Jurnal Ilmiah Aplikasi Isotop dan Radiasi, 6(1), 35–42. https://doi.org/10.17146/jair.2010.6.1.377
Ezward, C., Suliansyah, I., Rozen, N., & Dwipa, I. (2020). Identifikasi karakter vegetatif beberapa genotipe padi lokal kabupaten kuantan singingi. Menara Ilmu: Jurnal Penelitian dan Kajian Ilmiah, 14(2).
Farooq, M., Kobayashi, N., Ito, O., Wahid, A., & Serraj, R. (2010). Broader leaves result in better performance of indica rice under drought stress. Journal of plant physiology, 167(13), 1066-1075.
Forti, C. (2020). Seeds in the context of agricultural research: combining traditional and innovative approaches to boost seed quality. University of Pavia, Italy.
Ghosh, B., N. 1949. Physiological Studies On The Effect Of Colchicine On Rice II. Department Of Botany. Calcutta University. 3(16):135-145.
Gui, M. Y., Wang, D., Xiao, H. H., Tu, M., Li, F. L., Li, W. C., ... & Li, J. Y. (2018). Studies of the relationship between rice stem composition and lodging resistance. The Journal of Agricultural Science, 156(3), 387-395.
Hattori, Y., Nagai, K., Furukawa, S., Song, X. J., Kawano, R., Sakakibara, H., ... & Ashikari, M. (2009). The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water. Nature, 460(7258), 1026-1030.
Hu, Y., Li, J., Yang, L., Nan, W., Cao, X., & Bi, Y. (2014). Inhibition of root growth by narciclasine is caused by DNA damage-induced cell cycle arrest in lettuce seedlings. Protoplasma, 251, 1113-1124.
Husain I. Surdaya T, Purnomo S.H. 2022. Induksi Mutasi Menggunakan Kolkisin pada Umbi Bawang Merah (Allium ascalonicum L.) Varietas Tajuk. Jurnal Hort. Indonesia. 13(1): 1-7
Jordan, M. A., & Wilson, L. (2004). Microtubules as a target for anticancer drugs. Nature Reviews Cancer, 4(4), 253–265.
Kumar, A., Dixit, S., Ram, T., Yadaw, R. B., Mishra, K. K., & Mandal, N. P. (2014). Breeding high-yielding drought-tolerant rice: genetic variations and conventional and molecular approaches. Journal of experimental botany, 65(21), 6265-6278.
Manzoor, A., Ahmad, T., Bashir, M. A., Hafiz, I. A., & Silvestri, C. (2019). Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants, 8(7), 194.
Priyadi I. Get to know Rice Inpari Nutri Zinc (2021) http://cybex.pertanian.go.id/artikel/97852/
Sabana, A., Ernawiati, E., Priyambodo, P., & Agustrina, R. (2022). Induksi poliploid planlet pisang kepok batu dengan kolkisin pada media kultur jaringan. Organisms: Journal of Biosciences,2(1), 1-8.
Sahoo, S. A. (2018). Studies on Radiation Mutagenesis in Anther Cultures and Characterization of Induced Genetic Variability in Rice (Oryza sativa L.). Thesis, Odisha University of Agriculture and Technology.
Septiningsih, E. M., Pamplona, A. M., Sanchez, D. L., Neeraja, C. N., Vergara, G. V., Heuer, S., ... & Mackill, D. J. (2009). Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond. Annals of Botany, 103(2), 151-160.
Shu, G. Y. (2009). Induced plant mutations in the genomics era.
Singh, H. (2022). Morpho-physiological and molecular profiling of rice (Oryza sativa L.) genotypes for yield related traits under direct seeded conditions.KrishiKosh Repository, ICAR India.
Subekti, A., & Umar, A. (2023). Keragaman Enam Varietas Unggul Baru Padi Khusus Pada Lahan Sub Obtimal di Kalimatan Barat. Jurnal Pertanian Agros, 25(1), 514-520.
Suhartini, T., & Sutoro, S. (2007). Pengelompokan Plasmanutfah Spesies Padi Liar (Oryza spp.) Berdasarkan Peubah Kuantitatif Tanaman. Berita Biologi, 8(6), 445-453.
Sujipuli, K., Inthima, P., Yimtragool, N., Warnnissorn, N., Warnnissorn, P., & Prasarnpun, S. (2021). In vitro colchicine-induced polyploids from different explant segments of Bacopa monnieri. ScienceAsia, 47(1).
Sumiasih, I. H. (2014). Pengaruh Lama Perendaman Beberapa Varietas Padi dengan Colchicine terhadap Komponen Agronomi dan Produksi. Skripsi. Universitas Jember.
Surson, S., Sitthaphanit, S., Prachachit, J., Jitjak, T., & Wongkerson, K. (2024). Effects of colchicine on polyploid induction, morphology, and yield components of several Thai rice varieties. Biodiversitas Journal of Biological Diversity, 25(12).
Surson, S., Sitthaphanit, S., Prachachit, J., Jitjak, T., & Wongkerson, K. (2025). Effects of Colchicine on the Morphology and Agricultural Characteristics of RD. 43 Thai Rice Variety. Indian Journal of Agricultural Research, 59(1).
Wang, Y., Zhang, H., Gigant, B., Yu, Y., Wu, Y., Chen, X., ... & Knossow, M. (2016). Structures of a diverse set of colchicine binding site inhibitors in complex with tubulin provide a rationale for drug discovery. FEBS Journal, 283(1), 102-111.
Wu, L., Zhang, W., Ding, Y., Zhang, J., Cambula, E. D., Weng, F., ... & Li, G. (2017). Shading contributes to the reduction of stem mechanical strength by decreasing cell wall synthesis in japonica rice (Oryza sativa L.). Frontiers in plant science, 8, 881.
Yamin, M., & Sri Nur Qadri. (2023). Pendugaan Komponen Ragam dan Aksi Gen Karakter Agronomi Populasi F1 Kapas. Perbal: Jurnal Pertanian Berkelanjutan, 11(2), 238–245. https://doi.org/10.30605/perbal.v11i2.2773.
Zeinullina, A., Zargar, M., Dyussibayeva, E., Orazov, A., Zhirnova, I., Yessenbekova, G., ... & Hu, Y. G. (2023). Agro-morphological traits and molecular diversity of Proso Millet (Panicum miliaceum L.) affected by various colchicine treatments. Agronomy, 13(12), 2973.
Zhao, S., Jang, S., Lee, Y. K., Kim, D. G., Jin, Z., & Koh, H. J. (2020). Genetic basis of tiller dynamics of rice reveale
Published
2025-12-01
How to Cite
Hasan, E. N., Husain, I., Yamin, M., Rahim, Y., & Dama, H. (2025). Genetic Diversity of Agronomic Traits in Rice (Oryza sativa L.) Using Several Colchicine Concentrations. International Journal of Technology and Education Research, 3(04), 673-789. https://doi.org/10.63922/ijeter.v3i04.2522
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